Fanconi anemia (FA) is autosomal recessive disorder. Molecular study of FA revealed that there are 15 genetic subtypes groups with FANCA gene being the most frequent. The rate of successful cytogenetic cell culture varies with the specific type of disease. Many factors can affect the cytogenetic cell culture and the successful appearance of metaphase. The determination of the amount of blood uses in cytogenetic cell culture is important to obtain excellent metaphase. The highlight of the book lies on identification and diagnosis of Fanconi anemia among aplastic anemia patients with Special emphasis on identification of FANCA gene mutations. The book also improves the chromosomal breakage test and reduces lymphocyte cell culture failure. The book is extremely useful for medical laboratory students and researchers working in hematology. Postgraduate students would find this book informative and handy.
This book serves an approach towards fine mapping of Saltol QTL. Here a gene based molecular technique is used.Saltol QTL is an important gene responsible for the salinity tolerance of Rice. Salinity is a major cause for low production of rice in the coastal areas all over the world. In this book, we tried to develop a solution using molecular techniques which are fast and reliable. This books will help those who wants to widen their knowledge in advanced molecular research.
In Volume 2 of Essential Molecular Biology, procedures for preparing gene libraries and identifying genes are described, together with methods for studying the structure of a cloned gene and the way it is expressed in the cell.
Canine Distemper (CD) is a contagious, incurable, often fatal, multi-systemic viral disease that affects the respiratory, gastrointestinal, and central nervous systems. CD , caused by CD virus, is worldwide and has a wide host range such as canidae family (dog, fox ...etc). In this book; molecular and virological characterization of CDV Asian isolates were carried out, which introduced new biological and molecular aspects to these viruses. Such as; the ability of some isolates to propagate in Hamster cell lines, the role of fusion (F)gene or the presence of unidentified receptors other than well-known one can be used for the virus cell-interaction, the molecular properties of adapted viruses as well as the wild one and its role in virus virulence, the impact of F gene in lineages classification as hemagglutinin (H)gene.
Anemia is the most common blood disorder, and according to the National Heart, Lung, and Blood Institute, it affects more than 3 million Americans and an estimated 1.62 billion people in the world. Anemia is diagnosed as any condition in which our body does not produce enough healthy red blood cells. The condition has been discovered to date back to more than 4,000 years ago and is the most common blood disorder. Anemia is not strictly a disease, but a disorder. It is often a byproduct of other diseases that disrupt the body's ability to produce healthy red blood cells.Unfortunately, there is no one specific cause of anemia. Due to the sheer number of anemia types, it can sometimes be difficult to pinpoint the exact cause.A patient's diet can be a cause of anemia. A lack of iron or vitamin-rich foods severely impacts the body's capacity to produce enough healthy red blood cells.The most common symptom of anemia, regardless of type, is a feeling of fatigue and a lack of energy.Women who are menstruating or pregnant and people with chronic medical conditions are most at risk for this disease.
Leptin is a novel biomarker which is associated with milk production traits in dairy cattle. Leptin may prove to be most useful to animal producers as a stimulant for growth as well as reproductive traits or as a molecular marker to genetically select high performing individuals, ultimately with an aim to have higher production. Genetic polymorphisms in the intronic and exonic region of leptin gene reported are associated with productive and reproductive traits in cattle and this sort of genetic information can be used efficiently in breeding and management decisions. This monograph describe on the various aspect of bovine leptin gene in terms of molecular structure, mechanism of action, expression profile, polymorphic studies on leptin gene as well as the role of leptin for regulation of dairy milk production.
Breast cancer is the most common type of cancer among females, both in incidence and death. As meaningful biological understanding of the disease is confounded by the existence of various molecular groups and sub-groups, the challenge for targeted drug development may lie in understanding the molecular mechanisms of various sub-groups in breast cancer. This book describes the gene expression profiling approach for deeper understanding of the dynamics of breast cancer progression and prognosis.
Recent advances in genetic research have produced a surge of student interest in molecular biology/ molecular genetics courses. Molecular biology is a fast-paced field that constantly provides new insights into the living world and the role of humans within it. From a wide area of molecular biology, molecular genetics represents one of the major technological field of the twenty-first century. Its wide ranging, multi-disciplinary activities include polymerase cahin reactions, molecular marker based studies and the gene cloning. One of the main challenges for the instructor in such a course is to lacking of the proper and manageable practical handling during lab experimentation's.
Gene therapy is a gene or gene product that can be selectively delivered to a specific cell/tissue with minimal toxicity. Gene therapy is ‘the use of genes as medicine. It involves the transfer of a therapeutic or working gene copy into specific cells of an individual in order to repair a faulty gene copy. Thus it may be used to replace a faulty gene, or to introduce a new gene whose function is to cure or to favorably modify the clinical course of a condition. The scope of this new approach to the treatment of a condition is broad, with potential in the treatment of many genetic conditions, some forms of cancer and certain viral infections such as AIDS. Direct targeting of cancer cells with gene therapy has the potential to treat cancer on the basis of its molecular characteristics. But although laboratory results have been extremely encouraging, many practical obstacles need to be overcome before gene therapy can fulfill its goals in the clinic
Breast cancer is known as one of the most common causes of death among women worldwide. It is an aggressive type of cancer since it may invade other tissues in different ways. Breast cancer has been one of the major interests for research studies, especially in terms of gene profiling. Her-2/neu is a main gene to be studied in order to understand the status of the disease. Here in this book, Her-2/neu was evaluated using three different techniques; which revealed interesting results.
Sickle cell anemia is one of the major hereditary health problem in tropical Africa where the sickle cell gene frequency is particularly high. The disease occurs with high incidence in west,east and central Africa and this has been related to the prevalence of malaria in these regions. The classic hallmark of sickle cell anemia are episodes of vaso-occlusive crisis often with multiorgan manifestation and chronic haemolytic anemia. vaso-occlusive crisis is the most common cause of hospitalization in sickle cell disease. It affects the growth and academic performance of individual. Thus it would be quite helpful to terminate such painful episodes at a very early stage. Serum CPK,LDH and 24 hour urinary creatinine can be used as potential markers of tissue ischemia in such painful episodes.
This book is based on the role of co-morbid disorder; beta thalassemia minor with iron deficiency anemia in the promulgation of beta thalassemia gene. This book explain the pathophysiology of co-morbid condition and its effect on hematological parameters and Hb-A2 levels. This book is useful for the medical students, general physicians,consultant hematologist and Pathologist. In the areas where beta thalassemia is more common genetic disorder this book will proved as useful tool to stop the propagation of beta thalassemia gene in homozygous state.
Molecular markers are used for germplasm characterization, genetic mapping, gene tagging, and gene introgression from exotic species in brassica. Genetic maps aids to the understanding of genome in terms of structure, function and evolution and represents an important tool for crop enhancements on genetic level. Present study aims at reviewing the use of molecular markers for mapping and tagging qualitative and quantitative traits in brassica so that this information can be easily available to researchers as a reference. A number of important traits in light of molecular genetics in Brassica are described in detail.
The book embraces a number of areas of microbial genetics;many of which are new, whereas others carry on as strands from 2nd level microbiology, genetics and molecular biology courses. This book is intended for students interested in microbiology, molecular biology and genetics.The book covers fundamentally important.Topics may include genetics of bacteriophages,bacteria, archaea and yeast,mutation and evolution, mechanisms of gene transfer,gene regulation and adaptive responses,and genomics and functional genomics of individual microorganisms and whole microbial communities.
The association between Anemia of Chronic Disease (ACD)and chronic periodontitis has been studied with varying results .In the earlier studies it was reported that anemia plays a direct role in the etiology of periodontal disease . On the contrary, it has been demonstrated that anemia occurred as a result of destructive periodontal disease by means of chronic inflammation.This separate entity was termed as Anemia of Chronic Disease.Therefore in the present study, an attempt was made to explore a link between chronic periodontitis and anemia of chronic disease by evaluating certain hematologic markers indicative of ACD .