A microsomal membrane bound alcohol oxidase enzyme was isolated from a hydrocarbon degrading Aspergillus terreus fungus that could oxidize short chain-,long chain-, secondary-, and aryl-alcohol substrates. High aggregating property of the protein was demonstrated by AFM, DLS and TEM analyses. Chemical analysis showed the presence of oleic acid and palmitic acid at a ratio of 2:1 in the purified protein. We have demonstrated a highly efficient method for dissociation and simultaneous deflavination of the alcohol oxidase protein using ?-ME. The potential applications of this approach on preparing apoprotein and its reassemble with FAD to a functional enzyme are envisaged in the preparation of enzyme electrodes for biosensor and biofuel cell applications. The findings obtained through this investigation have revealed many interesting facts about this novel microsomal alcohol oxidase enzyme.This investigation has opened up new avenue on the research to explore the architecture of alcohol oxidase proteins from fungal sources including their structure-function relationship and potential applications.
As the topic says "ISOLATION, PRODUCTION, PURIFICATION, ASSAY& CHARACTERIZATION OF RECOMBINANT CHOLESTEROL OXIDASE FROM NATIVE Brevibacterium sp." The objective of this study is to isolate extracellular cholesterol oxidase (CHO) producing microorganisms to obtain an abundant source of cholesterol oxidase (CHO) for industrial and medicinal needs. Cholesterol oxidase (CHO) is an enzyme, which catalyzes the oxidation of cholesterol and converts 5-cholesten-3-ol into 4- cholesten-3-one. Cholesterol oxidase producing bacteria were isolated from waste of regional oil mill, soil and compost. Twenty-five isolates are tested for cholesterol oxidase activity by screening method. As the result of the screening, CHO producer strain was isolated and identified.Screening and isolation of bacterial strains producing extracellular form of cholesterol oxidase are very important due to its wide spectrum applications. Cholesterol oxidase enzyme has many applications in medicine, agriculture, industry and pharmaceutical sectors.
The first book of its kind to introduce the fundamentals, basic features and models, potential applications and novel phenomena and its important applications in liquid crystal technology. Recognized leader in the field Gaetano Assanto outlines the peculiar characteristics of nematicons and the promise they have for the future growth of this captivating new field.
The book addresses the basics, applications, and manufacturing of plasmid biopharmaceuticals. The survey of the most relevant characteristics of plasmids provides the basics for designing plasmid products (applications) and processes (manufacturing). Key features that the authors include in the book are: i) consistency and clear line of direction, ii) an extensive use of cross-referencing between the individual chapters, iii) a rational integration of chapters, iv) appellative figures, tables and schemes, and v) an updated, but selected choice of references, with a focus on key papers.
Cost-effective strategies for designing novel drug delivery systems that target a broad range of disease conditions In vivo imaging has become an important tool for the development of new drug delivery systems, shedding new light on the pharmacokinetics, biodistribution, bioavailability, local concentration, and clearance of drug substances for the treatment of human disease, most notably cancer. Written by a team of international experts, this book examines the use of quantitative imaging techniques in designing and evaluating novel drug delivery systems and applications. Drug Delivery Applications of Noninvasive Imaging offers a full arsenal of tested and proven methods, practices and guidance, enabling readers to overcome the many challenges in creating successful new drug delivery systems. The book begins with an introduction to molecular imaging. Next, it covers: In vivo imaging techniques and quantitative analysis Imaging drugs and drug carriers at the site of action, including low-molecular weight radiopharmaceuticals, peptides and proteins, siRNA, cells, and nanoparticles Applications of imaging techniques in administration routes other than intravenous injection, such as pulmonary and oral delivery Translational research leading to clinical applications Imaging drug delivery in large animal models Clinical applications of imaging techniques to guide drug development and drug delivery Chapters are based on a thorough review of the current literature as well as the authors' firsthand experience working with imaging techniques for the development of novel drug delivery systems. Presenting state-of-the-technology applications of imaging in preclinical and clinical evaluation of drug delivery systems, Drug Delivery Applications of Noninvasive Imaging offers cost-effective strategies to pharmaceutical researchers and students for developing drug delivery systems that accurately target a broad range of disease conditions.
This practical book summarizes the latest research results of orthogonally polarized lasers, birefringence laser cavities, and their applications. Coverage ranges from basic principles and technologies to the characteristics of different cavities and lasers to various measurement techniques. A number of figures, experimental designs, and measurement curves are included, helping readers gain a thorough understanding of the many applications in modern engineering and start their own projects. Many types of relevant lasers (Helium/Neon lasers, Nd:YAG lasers, laser diodes, etc.) are also discussed.
The NanoBMat company, GmbH - HRB132698 - is a nanotechnology research center. The main activity fields of this company are the study of novel nanoparticles, nanomaterials and nanocomposites for industrial different applications such as pharmacy, medical, aerotechnology, textile, food,…., the study of novel drug delivery systems for intelligent cancer therapy such as photodynamic therapy method and the study of novel conductive and superparamagnetic nanocomposites for aero- and pharmaceutical applications.
A multidisciplinary approach that explores the diverse properties, functions, and applications of nanomaterials Drawing together the many scientific and engineering disciplines underlying the development of nanomaterials, Nanoscale Multifunctional Materials provides a multidisciplinary review of the diverse properties, functions, and applications of nanomaterials. The book examines both nanoparticles, which have larger-scale equivalents, and uniquely assembled nanomaterials, which do not have larger-scale equivalents. Readers will gain a tremendous appreciation of the versatility of nanomaterials as well as an understanding of how the same nanomaterial can have several distinct applications across a broad range of fields and industries. Nanoscale Multifunctional Materials is divided into three sections: ? Section I, Overview, describes the scientific phenomena underlying the special properties of nanomaterials, making them desirable as novel materials and different from conventional solids. Next, readers will learn about the effect of nanomaterials on contemporary society as well as future trends in nanomaterials production and use. ? Section II, Processing and Analysis, explores several experimental approaches in nanomaterial fabrication and characterization as well as in theoretical approaches in modeling and simulation. ? Section III, Applications, offers detailed examples of nanomaterial applications in alternative energy, thermal management, environmental cleanup, water treatment, and biomedicine. Each chapter has been written by one or more leading experts in the science, engineering, and application of nanomaterials. Within each chapter, readers will find a thorough review of the current literature, with references to facilitate further investigation of individual topics. Underscoring the multidisciplinary and multifunctional characteristics of nanomaterials, this book is recommended for students and professionals in science and engineering who need a broad perspective on both the nature and application of nanomaterials. The text also sets the stage for the development of new nanomaterials and new applications.
This book focuses on the synthesis of novel representatives of sulfobetaine and carbobetaine polymers. Their synthesis, characterization and applications as photoluminescent material, biocompatible material and in analytical chemistry are presented here. A series of 7 copolymers is synthesized and presented in this book. Chapter 1 presents a relevant description of the “state of art” through literature survey. The synthesis, physical properties, characterization techniques and various experimental procedures are presented in chapter 2. Chapter 2 incorporates thermal degradation characteristics and antibacterial properties of these polymers. One of the sulfobetaine polymer showed photoluminescence characteristics which was studied in steady state as well with varying time domain and results are discussed in chapter 3. One of the polycarbobetaine was crosslinked with a diacrylate crosslinker and a series of gel was synthesized and studied in detail for their swelling characterization and their results are discussed in chapter 4. Chapter 5 and 6 deals with representatives of polybetaine tested as a novel polymer format for molecular imprinting of L-aspartic acid and baclofen drug.
Recent advances in digital media technologies have created a large number of secured multimedia applications. Those applications and services are often deployed in a wireless network environment. This book presents a new model for the three tier transcoding architecture. The objective of that model is to minimize the transcoding process while upholding the security aspect of the stream. In addition, a novel security technique is proposed. This technique is mainly designed to provide more security on higher levels of applications. A novel and simple scrambling method was proposed too. The results of implementing the developed security techniques according to the selective encryption equation of the off-line-scenario are deployed and analyzed.
Natural materials have attracted considerable attention for drug delivery applications due to their abundant availability, compatibility with environment and degradation under natural or physiological conditions. Natural gum resins have been used since ancient times for a wide range of applications: varnishes, sealant, binding media and waterproofing. The most commonly used resins for plant and varnishes are Rosin, Sandarac, Dammar, Mastic and Copal. Gum Dammar is a natural resinous material of plant Shorea javanica, family – Dipterocarpaceae. It contains about 40% alpha-resin (resin that dissolves in alcohol), 22% beta-resin, 23% dammarol acid and 2.5% water. This book deals with the research carried out on Dammar gum for its utility as pharmaceutical excipient. The various chapters of the book deals with characterization of dammar gum, its use as film forming, coating & microencapsulating agent, along with its biodegradation, biocompatibility and drug interaction studies.
Carbon nanotubes (CNTs) have novel properties that make them potentially useful in many applications in nanotechnology, electronics, optics and other fields of materials science. These characteristics include extraordinary strength, unique electrical properties, and the fact that they are efficient heat conductors. Field emission is the emission of electrons from the surface of a condensed phase into another phase due to the presence of high electric fields. CNT field emitters are expected to make a breakthrough in the development of field emission display technology and enable miniature X-ray sources that will find a wide variety of applications in electronic devices, industry, and medical and security examinations. This first monograph on the topic covers all aspects in a concise yet comprehensive manner – from the fundamentals to applications. Divided into four sections, the first part discusses the preparation and characterization of carbon nanotubes, while part two is devoted to the field emission properties of carbon nanotubes, including the electron emission mechanism, characteristics of CNT electron sources, and dynamic behavior of CNTs during operation. Part three highlights field emission from other nanomaterials, such as carbon nanowalls, diamond, and silicon and zinc oxide nanowires, before concluding with frontier R&D applications of CNT emitters, from vacuum electronic devices such as field emission displays, to electron sources in electron microscopes, X-ray sources, and microwave amplifiers. Edited by a pioneer in the field, each chapter is written by recognized experts in the respective fields.
In this work, intensive studies have been done to produce plasma beams that could be successfully used in a variety of applications. This thesis covers the design, construction and operation of a plasma beam. On the other side, some applications of the extracted beam with target materials were studied. To determine the plasma parameters, a single electrostatic probe with its electrical circuit are constructed and attached to the system. Accordingly, the argon plasma was obtained and its characteristics have been measured, for the sake to find out the most suitable ones to run successfully the possible aimed applications (cleaning, sputtering, coating and polymerization). This was made by plotting the plasma radial profile, the I-V characteristics and calculating the electron temperature and density at different pressures and electrodes`enter-spacings.
Zirconia-Erbium doped Fibers are a novel type of highly-doped fiber that allows high erbium ion concentrations to be realized in a short length of fiber without the detrimental effects of ion clustering and concentration quenching. The fiber has a multitude of novel applications, including the generation of compact optical amplifiers and single-longitudinal mode lasers. A particularly interesting application of the fiber is the development of compact fast and ultrafast pulse lasers together with carbon allotropes as passive saturable absorbers. This new systems will have high potential for a large number of real-world applications such as communications, manufacturing, medicine and defense.
Polypyrrole (PPy) as a conducting polymer has potential applications in electrical and electronic devices. This book therefore aimed to produce novel “polypyrrole fibres via the development of nanostructured conducting polypyrrole” by fibre spinning of PPy and to investigate the formed fibres for applications such as actuators, e-textiles, batteries, sensors and biomedical areas. As a result polypyrrole fibres have been produced for the first time. Subsequent work sought to improve the properties of these first generation PPy fibres by increasing the molecular weight, addition of carbon nanotubes (CNTs) and addition of a supporting polymer (alginate). The use of the host polymer also enabled a new fibre spinning method to be developed that included an in situ polymerization process. The highest conductivity and Young''s modulus of any conducting polymer based fibre reported to date was obtained by incorporating PPy into a CNT yarn. In summary, a range of novel fibres PPy materials have been developed for possible use in applications such as actuators, sensors, artificial muscles, batteries and biomedical applications.